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Some thoughts about lightning talks: It's not about cramming as much information as possible in there. I look at it as an invitation for others to see you out and chat.
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So oftentimes, I ask myself what is the most intriguing thing about my work that will make people curious about it.
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If you have a figure that summarizes your research and workflow, use that to drive your talk. Again, work backwards and prune mercilessly.
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If you're looking to work with people on a project, say that. If you want people to give you feedback on a project, ask that. Having an action or an ask that you want people to take is a powerful driver of a lightning talk.
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Science is getting more and more collaborative. It doesn't need to be a lonely pursuit. Doing a lightning talk could be the thing that gets you to think outside the box.
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When you make your work accessible and understandable to an audience outside of your field. the gratitude of your presenters can be a powerful driver of collaboration.
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Oops, sorry, I wrote this too quickly. I meant "audience members", not "presenters" here.